Description:
Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.
Subject:
DNA microarrays -- Research
Nucleic acid hybridization
Chromosome polymorphism
Biochemistry
Biophysics
Raw Url:
http://pdxscholar.library.pdx.edu/do/oai/?metadataPrefix=&verb=GetRecord&identifier=oai:pdxscholar.library.pdx.edu:chem_fac-1036
Source:
Chemistry Faculty Publications and Presentations
Repository Record Id:
oai:pdxscholar.library.pdx.edu:chem_fac-1036
Record Title:
Multiplex SNP Discrimination
https://pdxscholar.library.pdx.edu/chem_fac/37
info:doi/10.1529/biophysj.107.105320
https://pdxscholar.library.pdx.edu/context/chem_fac/article/1036/viewcontent/multiplex_snp_discrimination.pdf