Thirty-three isolates of Fusarium acuminatum obtained from inland Pacific Northwest forest nurseries were tested for their pathogenicity on young Douglas-fir germinants under controlled laboratory conditions. Tested isolates were from forest nursery soil, roots of healthy-appearing and diseased conifer seedlings, Styrofoam and hard plastic containers, conifer seeds, 411 and adult fungus gnats. The vast majority of isolates were non-pathogenic under test conditions. A few isolates from roots of healthy-appearing or diseased seedlings displayed low or moderate virulence. Although isolates of F. acuminatum are routinely encountered in forest nurseries, they are probably not important pathogens under most conditions and should usually not be considered a threat to seedling production.